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loading buffer  (Bio-Rad)


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    Bio-Rad loading buffer
    Loading Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 11874 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sample+loading+buffer/4x+Laemmli+Sample+Buffer/pm42897223-60-3-9
    Average 99 stars, based on 11874 article reviews
    loading buffer - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: NAD + repletion with niacin counteracts cancer cachexia
    Article Snippet: Total protein concentration was quantified with Bradford reagent (Bio-Rad) using BSA as protein concentration standard. .. Equal amounts of protein (10–30 μg) were heat-denatured (except when assessing OXPHOS expression) in sample-loading buffer (50 mM Tris-HCl, pH 6.8, 100 mM DTT, 2% SDS, 0.1% bromophenol blue, 10% glycerol), resolved by SDS-PAGE electrophoresis (4561086, Bio-Rad) and transferred to nitrocellulose membranes (1704159, Bio-Rad). .. Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (1:1000, 07-181, Millipore, polyclonal), p-AMPK Thr172 (1:1000, #2535, Cell Signaling, clone 40H9), GAPDH (1:10000, G8795, clone GAPDH-71.1), LC3B (1:1000, L7543, polyclonal), NRK2 (1:1000, produced in Dr. Gareth G Lavery’s lab, polyclonal), OXPHOS Antibody Cocktail (1:1000, ab110413, Abcam), PGC-1α (1:1000, AB3242, Merck Millipore, polyclonal), PINK1 (1:500, SAB2500794, polyclonal), Puromycin (1:1000, EQ0001, Kerafast, clone 3RH11), TOMM20 (1:1000, ab186735, Abcam, clone EPR15581-54) and Vinculin (1:2000, sc73614, Santa Cruz Biotechnology, clone 7F9).

    Article Title: NAD + repletion with niacin counteracts cancer cachexia.
    Article Snippet: Total protein concentration was quantified with Bradford reagent (Bio-Rad) using BSA as protein concentration standard. .. Equal amounts of protein (10–30μg) were heat-denatured (except when assessing OXPHOS expression) in sample-loading buffer (50mM Tris-HCl, pH 6.8, 100mM DTT, 2% SDS, 0.1% bromophenol blue, 10% glycerol), resolved by SDS-PAGE electrophoresis (4561086, Bio-Rad) and transferred to nitrocellulose membranes (1704159, Bio-Rad). .. Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (1:1000, 07-181,Millipore, polyclonal), p-AMPKThr172 (1:1000, #2535,Cell Signaling, clone 40H9), GAPDH (1:10000, G8795, clone GAPDH-71.1), LC3B (1:1000, L7543, polyclonal), NRK2 (1:1000, produced in Dr. Gareth G Lavery’s lab, polyclonal), OXPHOS Antibody Cocktail (1:1000, ab110413, Abcam), PGC-1α (1:1000, AB3242, Merck Millipore, polyclonal), PINK1 (1:500, SAB2500794, polyclonal), Puromycin (1:1000, EQ0001, Kerafast, clone 3RH11), TOMM20 (1:1000, ab186735, Abcam, clone EPR15581-54) and Vinculin (1:2000, sc73614, Santa Cruz Biotechnology, clone 7F9).

    SDS Page:

    Article Title: NAD + repletion with niacin counteracts cancer cachexia
    Article Snippet: Total protein concentration was quantified with Bradford reagent (Bio-Rad) using BSA as protein concentration standard. .. Equal amounts of protein (10–30 μg) were heat-denatured (except when assessing OXPHOS expression) in sample-loading buffer (50 mM Tris-HCl, pH 6.8, 100 mM DTT, 2% SDS, 0.1% bromophenol blue, 10% glycerol), resolved by SDS-PAGE electrophoresis (4561086, Bio-Rad) and transferred to nitrocellulose membranes (1704159, Bio-Rad). .. Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (1:1000, 07-181, Millipore, polyclonal), p-AMPK Thr172 (1:1000, #2535, Cell Signaling, clone 40H9), GAPDH (1:10000, G8795, clone GAPDH-71.1), LC3B (1:1000, L7543, polyclonal), NRK2 (1:1000, produced in Dr. Gareth G Lavery’s lab, polyclonal), OXPHOS Antibody Cocktail (1:1000, ab110413, Abcam), PGC-1α (1:1000, AB3242, Merck Millipore, polyclonal), PINK1 (1:500, SAB2500794, polyclonal), Puromycin (1:1000, EQ0001, Kerafast, clone 3RH11), TOMM20 (1:1000, ab186735, Abcam, clone EPR15581-54) and Vinculin (1:2000, sc73614, Santa Cruz Biotechnology, clone 7F9).

    Article Title: NAD + repletion with niacin counteracts cancer cachexia.
    Article Snippet: Total protein concentration was quantified with Bradford reagent (Bio-Rad) using BSA as protein concentration standard. .. Equal amounts of protein (10–30μg) were heat-denatured (except when assessing OXPHOS expression) in sample-loading buffer (50mM Tris-HCl, pH 6.8, 100mM DTT, 2% SDS, 0.1% bromophenol blue, 10% glycerol), resolved by SDS-PAGE electrophoresis (4561086, Bio-Rad) and transferred to nitrocellulose membranes (1704159, Bio-Rad). .. Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (1:1000, 07-181,Millipore, polyclonal), p-AMPKThr172 (1:1000, #2535,Cell Signaling, clone 40H9), GAPDH (1:10000, G8795, clone GAPDH-71.1), LC3B (1:1000, L7543, polyclonal), NRK2 (1:1000, produced in Dr. Gareth G Lavery’s lab, polyclonal), OXPHOS Antibody Cocktail (1:1000, ab110413, Abcam), PGC-1α (1:1000, AB3242, Merck Millipore, polyclonal), PINK1 (1:500, SAB2500794, polyclonal), Puromycin (1:1000, EQ0001, Kerafast, clone 3RH11), TOMM20 (1:1000, ab186735, Abcam, clone EPR15581-54) and Vinculin (1:2000, sc73614, Santa Cruz Biotechnology, clone 7F9).

    Article Title: Studying the Association of TKS4 and CD2AP Scaffold Proteins and Their Implications in the Partial Epithelial–Mesenchymal Transition (EMT) Process
    Article Snippet: .. Sample loading buffer (4.2 mL H 2 O, 1 mL 6.8 pH Tris, 2.8 mL glycerol, 1.6 mL 10% SDS, 0.4 mL β-mercaptoethanol, 1 mg bromophenol blue) was added to the supernatants, followed by incubation at 95 °C for 5 min. Each protein sample was subjected to 10% SDS-PAGE gel (BioRad, Berkeley, CA, USA) and blotted. ..

    Article Title: Insulin receptor substrate 1 is a novel member of EGFR signaling in pancreatic cells.
    Article Snippet: Pancreatic ductal adenocarcinoma is an extremely incurable cancer type characterized by cells with highly proliferative capacity and resistance against the current therapeutic options.. Our study reveals that IRS1 acts as a bridging molecule between EGFR and IGFR/InsR signalization providing a potential mechanism for the interplay between signaling pathways and bypassing EGFR-targeted or IGFR/InsR-targeted therapies.. The analysis of IRS1 phosphorylation status in four pancreatic cell lines identified the impact of EGFR signaling on IRS1 activation in comparison with InsR/IGFR signaling.

    Article Title: Extracellular laccase activity in engineered Synechocystis sp. PCC 6803 for mediator-free textile dye decolorization.
    Article Snippet: .. The extracted soluble proteins were then denatured by the addition of SDS-PAGE sample loading buffer (Laemmli 6x) containing 2- Mercaptoethanol (1:10), and heated at 95 ◦C for 10 min. Proteins were separated on 4–20 % SDS-PAGE (Mini-PROTEAN TGX Stain-FreeTM pre-cast gel, Bio-Rad) at 200 V for 30 min. .. The gel was imaged using a ChemiDoc MP System (Bio-Rad).

    Article Title: Studying the Association of TKS4 and CD2AP Scaffold Proteins and Their Implications in the Partial Epithelial-Mesenchymal Transition (EMT) Process.
    Article Snippet: .. Sample loading buffer (4.2 mL H2O, 1 mL 6.8 pH Tris, 2.8 mL glycerol, 1.6 mL 10% SDS, 0.4 mL β-mercaptoethanol, 1 mg bromophenol blue) was added to the supernatants, followed by incubation at 95 ◦C for 5 min. Each protein sample was subjected to 10% SDS-PAGE gel (BioRad, Berkeley, CA, USA) and blotted. ..

    Electrophoresis:

    Article Title: NAD + repletion with niacin counteracts cancer cachexia
    Article Snippet: Total protein concentration was quantified with Bradford reagent (Bio-Rad) using BSA as protein concentration standard. .. Equal amounts of protein (10–30 μg) were heat-denatured (except when assessing OXPHOS expression) in sample-loading buffer (50 mM Tris-HCl, pH 6.8, 100 mM DTT, 2% SDS, 0.1% bromophenol blue, 10% glycerol), resolved by SDS-PAGE electrophoresis (4561086, Bio-Rad) and transferred to nitrocellulose membranes (1704159, Bio-Rad). .. Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (1:1000, 07-181, Millipore, polyclonal), p-AMPK Thr172 (1:1000, #2535, Cell Signaling, clone 40H9), GAPDH (1:10000, G8795, clone GAPDH-71.1), LC3B (1:1000, L7543, polyclonal), NRK2 (1:1000, produced in Dr. Gareth G Lavery’s lab, polyclonal), OXPHOS Antibody Cocktail (1:1000, ab110413, Abcam), PGC-1α (1:1000, AB3242, Merck Millipore, polyclonal), PINK1 (1:500, SAB2500794, polyclonal), Puromycin (1:1000, EQ0001, Kerafast, clone 3RH11), TOMM20 (1:1000, ab186735, Abcam, clone EPR15581-54) and Vinculin (1:2000, sc73614, Santa Cruz Biotechnology, clone 7F9).

    Article Title: NAD + repletion with niacin counteracts cancer cachexia.
    Article Snippet: Total protein concentration was quantified with Bradford reagent (Bio-Rad) using BSA as protein concentration standard. .. Equal amounts of protein (10–30μg) were heat-denatured (except when assessing OXPHOS expression) in sample-loading buffer (50mM Tris-HCl, pH 6.8, 100mM DTT, 2% SDS, 0.1% bromophenol blue, 10% glycerol), resolved by SDS-PAGE electrophoresis (4561086, Bio-Rad) and transferred to nitrocellulose membranes (1704159, Bio-Rad). .. Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (1:1000, 07-181,Millipore, polyclonal), p-AMPKThr172 (1:1000, #2535,Cell Signaling, clone 40H9), GAPDH (1:10000, G8795, clone GAPDH-71.1), LC3B (1:1000, L7543, polyclonal), NRK2 (1:1000, produced in Dr. Gareth G Lavery’s lab, polyclonal), OXPHOS Antibody Cocktail (1:1000, ab110413, Abcam), PGC-1α (1:1000, AB3242, Merck Millipore, polyclonal), PINK1 (1:500, SAB2500794, polyclonal), Puromycin (1:1000, EQ0001, Kerafast, clone 3RH11), TOMM20 (1:1000, ab186735, Abcam, clone EPR15581-54) and Vinculin (1:2000, sc73614, Santa Cruz Biotechnology, clone 7F9).

    Article Title: Insights into cellular behavior and micromolecular communications in urothelial micrografts
    Article Snippet: Total protein concentration was evaluated using the bicinchoninic acid (BCA) assay as per manufacturer’s instructions (Thermo Fisher Scienti c, Waltham US). .. Fifty micrograms of total protein were heatdenatured in sample-loading buffer (4x Laemmli sample buffer, Bio-Rad Laboratories, Hercules US) and loaded into 7.5% SDS-polyacrylamide (SDS-PAGE) electrophoresis gel (Mini-PROTEAN® TGXTM Precast Gels, Bio-Rad) and then transferred onto polyvinylidene uoride (PVDF) membranes (Trans-Blot Turbo Transfer System, Bio-Rad). .. The membranes were thereafter blocked with Tris-buffered saline (TBS) containing 5% bovine serum albumin (BSA) and incubated over night with a 1:1000 dilution of primary Page 11/26 antibodies speci c for either ERK1/2 (rabbit ERK1/ERK2 monoclonal antibody, cat#MA5-15134, Invitrogen) or pERK1/2 (rabbit phospho-ERK1/ERK2(Thr185, Tyr187) polyclonal antibody, cat#44-680G, Invitrogen).

    other:

    Article Title: PSME3 Regulates Migration and Differentiation of Myoblasts
    Article Snippet: Primary antibodies were added directly into the clarified supernatant at 2.8 ug per 1e6 cells, with an isotype IgG (Biotechne, AB-105-C) used as a control.

    Incubation:

    Article Title: Studying the Association of TKS4 and CD2AP Scaffold Proteins and Their Implications in the Partial Epithelial–Mesenchymal Transition (EMT) Process
    Article Snippet: .. Sample loading buffer (4.2 mL H 2 O, 1 mL 6.8 pH Tris, 2.8 mL glycerol, 1.6 mL 10% SDS, 0.4 mL β-mercaptoethanol, 1 mg bromophenol blue) was added to the supernatants, followed by incubation at 95 °C for 5 min. Each protein sample was subjected to 10% SDS-PAGE gel (BioRad, Berkeley, CA, USA) and blotted. ..

    Article Title: Insulin receptor substrate 1 is a novel member of EGFR signaling in pancreatic cells.
    Article Snippet: Pancreatic ductal adenocarcinoma is an extremely incurable cancer type characterized by cells with highly proliferative capacity and resistance against the current therapeutic options.. Our study reveals that IRS1 acts as a bridging molecule between EGFR and IGFR/InsR signalization providing a potential mechanism for the interplay between signaling pathways and bypassing EGFR-targeted or IGFR/InsR-targeted therapies.. The analysis of IRS1 phosphorylation status in four pancreatic cell lines identified the impact of EGFR signaling on IRS1 activation in comparison with InsR/IGFR signaling.

    Article Title: Studying the Association of TKS4 and CD2AP Scaffold Proteins and Their Implications in the Partial Epithelial-Mesenchymal Transition (EMT) Process.
    Article Snippet: .. Sample loading buffer (4.2 mL H2O, 1 mL 6.8 pH Tris, 2.8 mL glycerol, 1.6 mL 10% SDS, 0.4 mL β-mercaptoethanol, 1 mg bromophenol blue) was added to the supernatants, followed by incubation at 95 ◦C for 5 min. Each protein sample was subjected to 10% SDS-PAGE gel (BioRad, Berkeley, CA, USA) and blotted. ..

    Staining:

    Article Title: Extracellular laccase activity in engineered Synechocystis sp. PCC 6803 for mediator-free textile dye decolorization.
    Article Snippet: .. The extracted soluble proteins were then denatured by the addition of SDS-PAGE sample loading buffer (Laemmli 6x) containing 2- Mercaptoethanol (1:10), and heated at 95 ◦C for 10 min. Proteins were separated on 4–20 % SDS-PAGE (Mini-PROTEAN TGX Stain-FreeTM pre-cast gel, Bio-Rad) at 200 V for 30 min. .. The gel was imaged using a ChemiDoc MP System (Bio-Rad).



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